| Title | Method for producing cis-unsaturated fatty acid by recombinant Candida rugosa lipase 1 (rCRL1) |
| Year | 2022 |
| Country | TW、US |
| Inventor | Lee, Guan-Chiun |
| Co-inventor | Kuo, Ting-Chun;Chen, Shun-Niang;Lin, Chia-Chi |
| Cert. No. | I 757901、US 11,685,939 B2 |
| Introduction | A method for producing cis-unsaturated fatty acid includes the operations below. (i) An oil-water mixture is provided, wherein the oil-water mixture includes 1 to 10 parts by weight of oil and 1 part by weight of water. (ii) 0.002 to 0.5 parts by weight of a recombinant Candida rugosa lipase 1 (rCRL1) is added into the oil-water mixture. (iii) The oil-water mixture is emulsified. (iv) The emulsified oil-water mixture is hydrolyzed and fatty acid is generated. (v) Oil-water is separated at a temperature of 55° C. to 65° C. and an oil phase layer is extracted. (vi) The cooling and filtering step is performed to obtain cis-unsaturated fatty acid. |
| Validity | 2041/08/27 |
| Domain | Food_Science_&_Technology |
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