| Title | Method for producing cis-unsaturated fatty acid by recombinant candida rugosa lipase |
| Year | 2024 |
| Country | CN |
| Inventor | LEE, GUAN CHIUN |
| Cert. No. | CN 114317625 B |
| Introduction | The method for producing cis-unsaturated fatty acid comprises the following operations. (i) An oil-water mixture is provided, wherein the oil-water mixture comprises 1 to 10 parts by weight of grease and 1 part by weight of water. (ii) Adding 0.002-0.5 weight part of recombinant candida rugosa lipase 1 (rCRL 1) into the oil-water mixed solution. (iii) emulsifying the oil-water mixture. (iv) And (3) carrying out hydrolysis reaction on the emulsified oil-water mixture to generate fatty acid. (v) Oil-water separation is carried out at a temperature of 55 ℃ to 65 ℃ and an oil phase layer is extracted. (vi) And cooling and filtering the oil phase layer to obtain cis-unsaturated fatty acid. |
| Validity | 2040/09/30 |
| Domain | Chemical Engineering |
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