Title Method for producing cis-unsaturated fatty acid by recombinant candida rugosa lipase
Year 2024
Country CN
Inventor LEE, GUAN CHIUN
Cert. No. CN 114317625 B
Introduction The method for producing cis-unsaturated fatty acid comprises the following operations. (i) An oil-water mixture is provided, wherein the oil-water mixture comprises 1 to 10 parts by weight of grease and 1 part by weight of water. (ii) Adding 0.002-0.5 weight part of recombinant candida rugosa lipase 1 (rCRL 1) into the oil-water mixed solution. (iii) emulsifying the oil-water mixture. (iv) And (3) carrying out hydrolysis reaction on the emulsified oil-water mixture to generate fatty acid. (v) Oil-water separation is carried out at a temperature of 55 ℃ to 65 ℃ and an oil phase layer is extracted. (vi) And cooling and filtering the oil phase layer to obtain cis-unsaturated fatty acid.
Validity 2040/09/30
Domain Chemical Engineering
View count: 137