Title | Method for extracting IgY (γ-livetin) from egg yolk |
Year | 2017 |
Country | TW、US |
Inventor | WANG, YU CHIE |
Co-inventor | WANG, ZONG MING |
Cert. No. | I460189、US9,605,052B2 |
Introduction | A method for extracting IgY (γ-livetin) from yolk is disclosed, which comprises the following steps: (A) providing a buffer solution, a yolk sample, and an inorganic salt solution; (B) diluting the yolk sample with the buffer solution to obtain a mixture, stirring the mixture for a predetermined time, and performing a centrifugation on the mixture to obtain a supernatant; and (C) adding the inorganic salt solution into the supernatant to salt out IgY, wherein a pH value of the buffer solution is in a range from 4.6 to 5.4, a salt concentration of the buffer solution is in a range from 0.05 M to 0.15 M, and a saturation degree of the inorganic salt solution is in a range from 30% to 60%. |
Validity | 2033/09/19 |
Domain | Medicine_and_Health |
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